Serological and Molecular Analysis of the Results of Medical Staff Vaccination Against Hepatitis | CMAC

Serological and Molecular Analysis of the Results of Medical Staff Vaccination Against Hepatitis

Clinical Microbiology and Antimicrobial Chemotherapy. 2015; 17(1):73-78

​ Semenenko T.A., Nikitina G.Yu., Yarosh L.V., Bazhenov A.I., Elgort D.A., Kleymenov D.A., Tanicheva V.B., Godkov M.A., Suslov A.P.
hepatitis B virus medical staff vaccination against hepatitis B vaccine-induced immunity HB-mutants
Section
Personal Experience
Type
Journal article
Publication
Clinical Microbiology and Antimicrobial Chemotherapy. 2015; 17(1):73-78

Abstract

The results of the immune response intensity and duration in 890 hospital staff after vaccination against hepatitis B are presented. High frequency of HBsAg (1,7%), anti-HBs (21,1%), anti-HВe (16,9%) among not vaccinated against hepatitis B medical staff in comparison with vaccinated persons (0,2, 10,7 and 6,4% respectively) was established. «Isolated» anti-HBc was estimated to occur at 5 (0,8%) vaccinated and 15 (6,3%) not vaccinated persons, HBV DNA — at 3 (0,5%) and 5 (2,1%), respectively. Among 653 vaccinated medical staff antiHBs were absent or their concentration was below protective level at 140 (21,4%) persons. HBV DNA positive in PCR isolates had genotype D, subtypes ayw3 and ayw2. Sequence analysis of PCR products amplified from the S region revealed site replacements T118A, T118V, M125T, A128V. The necessity of the new generation vaccines formation capable to protect from HBV «wild» strains and mutant forms is discussed.

Tatyana A. Semenenko

Research Institute of Epidemiology and Microbiology named after N. F. Gamaleya, Moscow, Russia

Galina Yu. Nikitina

City Clinical Hospital named after S.P. Botkin, Moscow, Russia

Yarosh L.V.

Research Institute of Epidemiology and Microbiology named after N. F. Gamaleya, Moscow, Russia

Bazhenov A.I.

Research Institute of Emergency Care named after N.V. Sklifosovskiy, Moscow, Russia

Elgort D.A.

Research Institute of Epidemiology and Microbiology named after N. F. Gamaleya, Moscow, Russia

Kleymenov D.A.

Research Institute of Emergency Care named after N.V. Sklifosovskiy, Moscow, Russia

Tanicheva V.B.

City Clinical Hospital named after S.P. Botkin, Moscow, Russia

Godkov M.A.

Research Institute of Emergency Care named after N.V. Sklifosovskiy, Moscow, Russia

Suslov A.P.

Research Institute of Epidemiology and Microbiology named after N. F. Gamaleya, Moscow, Russia

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