Molecular Typing of Legionella pneumophila and Legionella spp. Strains, isolated in the Russian Federation | CMAC

Molecular Typing of Legionella pneumophila and Legionella spp. Strains, isolated in the Russian Federation

Clinical Microbiology and Antimicrobial Chemotherapy. 2008; 10(2):154-162

Type
Journal article

Abstract

On the base of international standarts of molecular-genetic typing (SBT protocol and EQA scheme using DNA sequencing of the mip-gene of the European Working Group for Legionella Infections — EWGLI) were descibed the strains of Legionella pneumophila and Legionella species, isolated in the Russian Federation during the last years at the monitoring and at the epidemic outbreak, and strains, presented in Gamaleya Institute Collection of Legionellosis (GICL). It was determined that 31 new strains from 3 Russian Federation’s regions belonged to 19 allelic profiles. It was revealed, that profiles 1, 4, 3, 1, 1, 1, 6 и 6, 10, 19, 28, 19, 4, 3 were most prevalent among the native strains. 9 new allelic profiles were identified, which were not earlier submitted in EWGLI base. On the base of EQA scheme using sequencing of the mip-gene the species identification of three new Legionella’s strains were made: 2 — L. gratiana and 1 — L. gormanii. The identified strains were not tested in the RF’s territory earlier.

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